3HFQ
Crystal structure of the lp_2219 protein from Lactobacillus plantarum. Northeast Structural Genomics Consortium Target LpR118.
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-05-01 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97860, 0.97917, 0.91837 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 78.543, 54.029, 79.584 |
Unit cell angles | 90.00, 104.18, 90.00 |
Refinement procedure
Resolution | 31.447 - 1.963 |
R-factor | 0.17 |
Rwork | 0.169 |
R-free | 0.17800 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.475 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXDE |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 500.000 | 2.040 |
High resolution limit [Å] | 1.963 | 1.970 |
Rmerge | 0.041 | 0.074 |
Number of reflections | 87372 | |
<I/σ(I)> | 22.2 | 13.6 |
Completeness [%] | 96.7 | 90.4 |
Redundancy | 1.7 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microbatch under oil | 291 | 20% PEG 3350, 0.2M ammonium phosphate, Microbatch under oil, temperature 291K |