3HFH
Crystal structure of tandem FF domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2008-04-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 141.300, 141.300, 155.420 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.016 - 2.703 |
| R-factor | 0.2353 |
| Rwork | 0.230 |
| R-free | 0.28330 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.153 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.016 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.109 | 0.563 |
| Number of reflections | 44246 | |
| <I/σ(I)> | 34.703 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 11.7 | 11.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 50mM Bis-Tris pH 6.5, 50 mM Ammonium sulfate, 30% Pentaerythritol ethoxylate, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
