3HEQ
Human prion protein variant D178N with M129
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Collection date | 2003-03-18 |
| Wavelength(s) | 1.1000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 57.489, 57.489, 168.013 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.120 - 1.800 |
| R-factor | 0.207 |
| Rwork | 0.206 |
| R-free | 0.23200 |
| Structure solution method | SIR |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.243 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 3.880 | 1.800 |
| Rmerge | 0.052 | 0.042 | 0.187 |
| Number of reflections | 26006 | ||
| <I/σ(I)> | 36.939 | ||
| Completeness [%] | 95.8 | 98.5 | 80.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 0.1 M Tris, 0.2 M Mg acetate, 5% PEG4K, 5mM CdCl2, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
