3HEN
Ferric Horse Heart Myoglobin; H64V/V67R Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 104 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-01-04 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 30.323, 119.002, 57.775 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.090 - 1.900 |
| R-factor | 0.18301 |
| Rwork | 0.180 |
| R-free | 0.23322 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | : 3HC9 |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.919 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.400 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.076 | 0.462 |
| Number of reflections | 15394 | |
| <I/σ(I)> | 11 | 3.3 |
| Completeness [%] | 91.0 | 91.5 |
| Redundancy | 7.07 | 7.32 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 295 | ammonium phosphate, EDTA, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
