3HEF

Crystal structure of the bacteriophage Sf6 terminase small subunit

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-26
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.02462
Spacegroup name	P 4 21 2
Unit cell lengths	88.858, 88.858, 72.631
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.650
Rwork	0.224
R-free	0.25100
Structure solution method	MAD
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	RESOLVE (2.13)
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.710
High resolution limit [Å]	1.650	1.650
Rmerge	0.053	0.521
Number of reflections	35647
<I/σ(I)>	107.121
Completeness [%]	99.7	100
Redundancy	28.4	28.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	1.2M NaH2PO4/0.8M K2HPO4, 0.2M Li2SO4, 5% glycerol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7	293	1.2M NaH2PO4/0.8M K2HPO4, 0.2M Li2SO4, 5% glycerol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K