3HE8
Structural study of Clostridium thermocellum Ribose-5-Phosphate Isomerase B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6C1 |
| Synchrotron site | PAL/PLS |
| Beamline | 6C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-18 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9999 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 69.515, 69.515, 154.434 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.810 - 1.900 |
| R-factor | 0.18924 |
| Rwork | 0.188 |
| R-free | 0.22011 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vvr |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.332 |
| Phasing software | swiss-model |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.035 | 0.051 |
| Number of reflections | 32228 | |
| <I/σ(I)> | 63.1 | 63.1 |
| Completeness [%] | 92.2 | 92.2 |
| Redundancy | 21.1 | 21.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 283 | 0.05M Tris, pH7.0, 10% PEG8000, 0.15M Magnesium chloride, 0.2M Potassium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 283K |
