3HE5
Heterospecific coiled-coil pair SYNZIP2:SYNZIP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-04-19 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 31 |
| Unit cell lengths | 49.905, 49.905, 113.228 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.430 - 1.750 |
| R-factor | 0.192 |
| Rwork | 0.190 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.812 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.430 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.294 | |
| Number of reflections | 31354 | |
| <I/σ(I)> | 42.868 | |
| Completeness [%] | 98.2 | 90.7 |
| Redundancy | 4.6 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 45% MPD, 100mM TRIS, 160 mM ammonium acetate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
