3HE4
Heterospecific coiled-coil pair SYNZIP5:SYNZIP6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97927 |
| Spacegroup name | P 63 |
| Unit cell lengths | 82.678, 82.678, 150.545 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.090 - 2.460 |
| R-factor | 0.216 |
| Rwork | 0.212 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.681 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.090 | 2.550 |
| High resolution limit [Å] | 2.460 | 2.460 |
| Number of reflections | 21204 | |
| <I/σ(I)> | 26.346 | |
| Completeness [%] | 99.7 | 99.3 |
| Redundancy | 5.8 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 295 | 100 mM TRIS, 20% MPD, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
