3HE1
Secreted protein Hcp3 from Pseudomonas aeruginosa.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-17 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.9792 |
Spacegroup name | P 3 1 2 |
Unit cell lengths | 141.219, 141.219, 105.052 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.800 - 2.098 |
R-factor | 0.216 |
Rwork | 0.214 |
R-free | 0.25400 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.189 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXD |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.800 | 2.140 |
High resolution limit [Å] | 2.098 | 2.100 |
Rmerge | 0.120 | 0.647 |
Number of reflections | 69851 | |
<I/σ(I)> | 28.392 | 4.09 |
Completeness [%] | 100.0 | 100 |
Redundancy | 10.4 | 9.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 0.1 M Bis-Tris buffer, 3.2 M sodium chloride, chymotrypsin partial digestion in crystallization droplet, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |