3HE0
The Structure of a Putative Transcriptional Regulator TetR Family Protein from Vibrio parahaemolyticus.
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-03-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97942, 0.97931 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 70.272, 95.548, 190.541 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.670 - 2.200 |
R-factor | 0.189 |
Rwork | 0.187 |
R-free | 0.22700 |
Structure solution method | MAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.436 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.190 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.089 | 0.580 |
Number of reflections | 34848 | |
<I/σ(I)> | 33.882 | |
Completeness [%] | 99.7 | 99.9 |
Redundancy | 9.5 | 7.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.8M ammonium sulfate,0.1M tri-sodium citrate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |