3HDV
Crystal structure of response regulator receiver protein from Pseudomonas putida
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-15 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 110.769, 140.436, 48.571 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.500 - 2.090 |
R-factor | 0.207 |
Rwork | 0.205 |
R-free | 0.24500 |
Structure solution method | SAD |
RMSD bond length | 0.020 |
RMSD bond angle | 1.875 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXD |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.500 | 2.180 |
High resolution limit [Å] | 2.090 | 2.100 |
Rmerge | 0.096 | 0.520 |
Number of reflections | 45411 | |
<I/σ(I)> | 10.3 | 5 |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 14.2 | 13.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 298 | 0.1M HEPES pH7.5, 0.8M Potassium Sodium Tartarate tetrahydrate, VAPOR DIFFUSION, SITTING DROP, temperature 298K |