3HDG
Crystal structure of the N-terminal domain of an uncharacterized protein (WS1339) from Wolinella succinogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.331, 77.059, 75.087 |
| Unit cell angles | 90.00, 102.67, 90.00 |
Refinement procedure
| Resolution | 38.530 - 2.270 |
| Rwork | 0.223 |
| R-free | 0.27000 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX (& SHARP) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.220 | 2.350 |
| High resolution limit [Å] | 2.270 | 2.270 |
| Rmerge | 0.107 | 0.560 |
| Number of reflections | 24794 | |
| <I/σ(I)> | 10.6 | |
| Completeness [%] | 99.5 | 95.6 |
| Redundancy | 7.2 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 30% PEG MME 550, 50mM Magnesium Chloride, 100mM HEPES pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
