3HDD
ENGRAILED HOMEODOMAIN DNA COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 123 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-07-15 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 130.620, 45.520, 72.930 |
| Unit cell angles | 90.00, 118.32, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| R-free | 0.23200 |
| Starting model (for MR) | 1hdd |
| RMSD bond length | 0.008 |
| RMSD bond angle | 19.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.042 | 0.200 |
| Total number of observations | 47760 * | |
| Number of reflections | 18176 | |
| <I/σ(I)> | 25 | 4 |
| Completeness [%] | 94.0 | 70 |
| Redundancy | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | COMPLEX WAS CRYSTALLIZED BY HANGING DROP VAPOR DIFFUSION OVER 0.26M AMOAC AND 1% PEG400., pH 7.0, vapor diffusion - hanging drop |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | AMOAC | ||
| 2 | 1 | 1 | PEG 400 | ||
| 3 | 1 | 2 | PEG 400 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mM) | |
| 2 | 1 | drop | ammonium acetate | 1 (M) | |
| 3 | 1 | reservoir | PEG400 | 1 (%(v/v)) | |
| 4 | 1 | reservoir | ammonium acetate | 0.25 (M) |
