3HD4
MHV Nucleocapsid Protein NTD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-01-14 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.160, 46.864, 71.706 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.273 - 1.747 |
| R-factor | 0.189 |
| Rwork | 0.185 |
| R-free | 0.22600 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.087 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.300 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.049 | 0.405 |
| Number of reflections | 12133 | |
| Completeness [%] | 99.5 | 97.2 |
| Redundancy | 5.2 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 30% PEG 1000, 50mM CAPSO, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
