3HD0
Crystal structure of Tm1865, an Endonuclease V from Thermotoga Maritima
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-06-27 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97876, 0.97860, 0.97243 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 69.571, 71.966, 120.266 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.700 |
R-factor | 0.16742 |
Rwork | 0.163 |
R-free | 0.25518 |
Structure solution method | MAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.635 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE (2.13) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.131 | 0.453 |
Number of reflections | 17147 | |
<I/σ(I)> | 15.333 | 3.27 |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 5.3 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | Crystallization drop was 1:1 mixture of protein dissolved in 20 mM Tris pH 8.5, 100 mM NaCl and 24 w/v% PEG 1500, 20% glycerol. The crystallization reservoir was 1.5 M NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 298K |