3HCY
The crystal structure of the domain of putative two-component sensor histidine kinase protein from Sinorhizobium meliloti 1021
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794, 0.9796 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 73.346, 73.346, 257.258 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 61.660 - 2.800 |
| R-factor | 0.20969 |
| Rwork | 0.207 |
| R-free | 0.26018 |
| Structure solution method | MAD |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.052 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.660 | 2.873 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.105 | 0.840 |
| Number of reflections | 10322 | |
| <I/σ(I)> | 30.2 | 3.38 |
| Completeness [%] | 99.8 | 99.1 |
| Redundancy | 25.7 | 24.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 289 | 0.1M Sodium Acetate, 2.0M Sodium Formate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
