3HC7

Crystal structure of lysin B from Mycobacteriophage D29

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	120
Detector technology	CCD
Collection date	2009-02-11
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9795
Spacegroup name	P 43 21 2
Unit cell lengths	92.530, 92.530, 69.630
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.380 - 2.000
Rwork	0.205
R-free	0.25000
Structure solution method	SIRAS
RMSD bond length	0.004
RMSD bond angle	0.635
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	21027
Completeness [%]	99.8	99.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8	290	50mM Tris pH8, 20% PEG1000, 100mM Calcium Acetate, VAPOR DIFFUSION, HANGING DROP, temperature 290K