3HC0
BHA10 IgG1 wild-type Fab - antibody directed at human LTBR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-01 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 139.157, 46.193, 150.628 |
| Unit cell angles | 90.00, 96.48, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.900 |
| R-factor | 0.182 |
| Rwork | 0.180 |
| R-free | 0.20600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.448 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.064 | 0.373 |
| Number of reflections | 76203 | |
| Completeness [%] | 96.2 | 88.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 22% PEG 4000, 100 mM calcium acetate and 100 mM Tris at pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
