3HBV
PrtC methionine mutants: M226A in-house
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-10-20 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 0.812 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 101.961, 101.961, 123.222 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.346 - 1.950 |
| R-factor | 0.153 |
| Rwork | 0.153 |
| R-free | 0.16600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1go8 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.008 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | PHENIX (1.4_54) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.990 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.034 | 0.184 |
| Number of reflections | 54277 | |
| <I/σ(I)> | 3.773 | 7.5 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 6 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 2 M NaCl, 0.1 M phosphate, pH 6.5, vapor diffusion, hanging drop, temperature 293K |
