3HBM
Crystal Structure of PseG from Campylobacter jejuni
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12B |
Synchrotron site | NSLS |
Beamline | X12B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-04-28 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9791 |
Spacegroup name | P 41 |
Unit cell lengths | 93.647, 93.647, 42.702 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.110 - 1.800 |
R-factor | 0.1981 |
Rwork | 0.196 |
R-free | 0.23458 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.261 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXDE |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.110 | 1.870 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 34208 | |
<I/σ(I)> | 22.6 | 8.4 |
Completeness [%] | 98.8 | 97.7 |
Redundancy | 7.4 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1M Bis-Tris, 0.2M Ammonium sulfate, 23% (v/v) PEG monomethyl ether 550, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |