3HBF
Structure of UGT78G1 complexed with myricetin and UDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.166, 77.581, 59.554 |
| Unit cell angles | 90.00, 96.25, 90.00 |
Refinement procedure
| Resolution | 35.820 - 2.100 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.820 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.046 | 0.252 |
| Number of reflections | 25599 | |
| <I/σ(I)> | 25.4 | 3.6 |
| Completeness [%] | 98.4 | 90 |
| Redundancy | 3.7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 290 | 25% PEG 3350, 0.1M Bis-Tris, pH 5.5, VAPOR DIFFUSION, temperature 290K |
