3HAH
Crystal structure of human PACSIN1 F-BAR domain (C2 lattice)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2008-10-30 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 160.009, 85.457, 88.392 |
| Unit cell angles | 90.00, 117.56, 90.00 |
Refinement procedure
| Resolution | 48.070 - 2.770 |
| R-factor | 0.2169 |
| Rwork | 0.215 |
| R-free | 0.26010 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.998 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.070 | 3.000 |
| High resolution limit [Å] | 2.770 | 2.770 |
| <I/σ(I)> | 9.9 | 2.5 |
| Completeness [%] | 27.5 | |
| Redundancy | 2.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 0.2M potassium sodium tartrate tetrahydrate, 20% PEG 3350, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
