3HA4
Crystal structure of the type one membrane protein MIX1 from Leishmania
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-15 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.95364 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 92.524, 100.389, 140.651 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.961 - 2.400 |
| R-factor | 0.214 |
| Rwork | 0.212 |
| R-free | 0.26060 |
| Structure solution method | MIR |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.421 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.250 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.183 | 0.737 |
| Number of reflections | 90601 | |
| <I/σ(I)> | 3.64 | |
| Completeness [%] | 99.3 | 99.3 |
| Redundancy | 6.6 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 1.18M di-ammonium-hydrogen phosphate, 0.1M citric acid, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
