3H9Z
Crystal structure of the IgE-Fc3-4 domains
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 5ID-B |
Synchrotron site | APS |
Beamline | 5ID-B |
Temperature [K] | 113 |
Detector technology | CCD |
Collection date | 1999-08-07 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 1.0001 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 65.660, 99.352, 77.639 |
Unit cell angles | 90.00, 97.40, 90.00 |
Refinement procedure
Resolution | 28.710 - 2.450 |
R-factor | 0.23182 |
Rwork | 0.229 |
R-free | 0.27748 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fp5 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.448 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.540 |
High resolution limit [Å] | 2.450 | 2.450 |
Number of reflections | 36017 | |
<I/σ(I)> | 18.425 | |
Completeness [%] | 98.2 | 82.3 |
Redundancy | 3.63 | 2.28 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 293 | 1 microliter of protein at 10 mg/mL in 20 mM sodium chloride was added to 1 microliter of well solution (100 mM ammonium acetate, 100 mM sodium acetate pH 4.6, 30% (w/v) PEG 4000) and mixed by pipetting., VAPOR DIFFUSION, HANGING DROP, temperature 293K |