3H9N

Crystal structure of the ribosome maturation factor rimm (hi0203) from h.influenzae. northeast structural genomics consortium target IR66.

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-04-02
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97901 0.97927 0.96790
Spacegroup name	I 21 3
Unit cell lengths	129.642, 129.642, 129.642
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.700
Rwork	0.211
R-free	0.23700
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.300
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.091	0.523
Number of reflections	19211
<I/σ(I)>	46.3	5
Completeness [%]	99.8	100
Redundancy	34.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6		0.4M Li2SO4, 0.1M Na3Citrate, 0.2M NH42SO4, ph 5.6, VAPOR DIFFUSION, HANGING DROP