3H9H
Human Class I MHC HLA-A2(A150P) in complex with the Tel1p peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-19 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.155, 86.885, 79.409 |
| Unit cell angles | 90.00, 89.97, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.174 |
| Rwork | 0.171 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tvb |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.632 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.069 | 0.316 |
| Number of reflections | 56073 | |
| <I/σ(I)> | 16.54 | 2.52 |
| Completeness [%] | 97.3 | 86.3 |
| Redundancy | 3.6 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | PEG3350 24%, MES 0.025M, NaF 0.1M, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
