3H9B
Structure of a mutant methionyl-tRNA synthetase with modified specificity complexed with azidonorleucine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-13 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.259, 45.377, 85.890 |
| Unit cell angles | 90.00, 107.24, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.500 |
| R-factor | 0.165 |
| Rwork | 0.165 |
| R-free | 0.18800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3h97 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.216 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.600 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.026 | 0.094 |
| Number of reflections | 86282 | 15200 |
| <I/σ(I)> | 27.6 | 10.1 |
| Completeness [%] | 93.1 | 93.9 |
| Redundancy | 3.1 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.1 | 279 | Ammonium citrate, pH 7.1, VAPOR DIFFUSION, SITTING DROP, temperature 279K |
