3H96
Msmeg_3358 F420 Reductase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979461 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.650, 71.150, 63.840 |
| Unit cell angles | 90.00, 90.39, 90.00 |
Refinement procedure
| Resolution | 45.700 - 2.000 |
| R-factor | 0.18939 |
| Rwork | 0.185 |
| R-free | 0.25508 |
| Structure solution method | SAD |
| RMSD bond length | 0.025 |
| RMSD bond angle | 1.848 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.510 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.105 | 0.174 |
| Number of reflections | 34129 | |
| <I/σ(I)> | 12.1 | 7.2 |
| Completeness [%] | 94.5 | 91.5 |
| Redundancy | 7.2 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277.15 | 36 w/v polyethylene glycol monomethyl ether 5000, 0.1 M sodium acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |
