3H94
Crystal structure of the membrane fusion protein CusB from Escherichia coli
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.3779,0.9792,0.9793,0.9949 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 85.005, 114.418, 259.080 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.200 - 3.840 |
| R-factor | 0.28 |
| Rwork | 0.280 |
| R-free | 0.30000 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.980 |
| High resolution limit [Å] | 3.840 | 3.840 |
| Rmerge | 0.072 | 0.412 |
| Number of reflections | 363533 | |
| <I/σ(I)> | 24 | 7.5 |
| Completeness [%] | 99.4 | 95.2 |
| Redundancy | 3.8 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.6 | 298 | 15%PEG1000, 360mM Lithium Citrate, 5% Glycerol, 5% Isopropanol, pH 5.6, VAPOR DIFFUSION, temperature 298K |
