3H93
Crystal Structure of Pseudomonas aeruginosa DsbA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-03-08 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.95364 |
Spacegroup name | P 41 |
Unit cell lengths | 41.155, 41.155, 98.318 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.106 - 1.501 |
R-factor | 0.112 |
Rwork | 0.110 |
R-free | 0.15200 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.969 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | RESOLVE (2.13) |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.076 | 0.361 |
Number of reflections | 51579 | |
<I/σ(I)> | 46.483 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 28 | 17.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 24% w/v PEG 1500, 22% v/v Glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |