3H8Y
Crystal structure of carboxysome small shell protein CsoS1C from Halothiobacillus neapolitanus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 6 |
| Unit cell lengths | 66.675, 66.675, 63.007 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.240 - 2.510 |
| R-factor | 0.258 |
| Rwork | 0.255 |
| R-free | 0.31100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g13 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.909 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (1.3.2) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.000 | 2.690 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.186 | 0.359 |
| Number of reflections | 5538 | |
| <I/σ(I)> | 13.17 | 6.94 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.3 | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.1M Tris HCl pH 8.5, 0.15M Sodium Citrate, 25% PEG 400, 0.01M nickel (II) chloride, vapor diffusion, hanging drop, temperature 298K |
