3H8W
Structure of D132N T4 RNase H in the presence of divalent magnesium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.629, 74.572, 88.242 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.340 - 2.800 |
R-factor | 0.231 |
Rwork | 0.229 |
R-free | 0.28700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1TFR native T4 RNase H |
RMSD bond length | 0.002 |
RMSD bond angle | 0.498 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.25) |
Phasing software | AMoRE |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.340 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.041 | 0.177 |
Total number of observations | 4280 | |
Number of reflections | 8890 | |
<I/σ(I)> | 15.3 | 4.3 |
Completeness [%] | 97.4 | 94.5 |
Redundancy | 3.6 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 100 mM Na PIPES, 200 mM MgCl2, 14% PEG 8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |