3H8M
SAM domain of human ephrin type-a receptor 7 (EPHA7)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-04-03 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.071, 54.089, 40.455 |
| Unit cell angles | 90.00, 104.84, 90.00 |
Refinement procedure
| Resolution | 22.240 - 2.100 |
| R-factor | 0.19867 |
| Rwork | 0.197 |
| R-free | 0.22767 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b4f |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.245 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0063) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.240 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 9717 | |
| <I/σ(I)> | 25.4 | 2.59 |
| Completeness [%] | 97.4 | 81.1 |
| Redundancy | 3.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293.1 | WELL SOLUTION: 20% PEG 4000, 0.2 M SODIUM ACETATE, 0.1 M TRIS HCL PH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.1K |
