3H8H

Structure of the C-terminal domain of human RNF2/RING1B;

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-03-07
Detector	RIGAKU RAXIS IV
Spacegroup name	P 65 2 2
Unit cell lengths	72.169, 72.169, 90.966
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.280 - 2.000
R-factor	0.202
Rwork	0.200
R-free	0.24400
Structure solution method	SAD
RMSD bond length	0.018
RMSD bond angle	1.702
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.5.0063)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.280	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	9884
<I/σ(I)>	28.58	1.67
Completeness [%]	99.4	99.9
Redundancy	13.2	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5	289	2 M AMMONIUM SULPHATE, 2% PEG 400, 0.1 M HEPES, PH 7.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 289K