3H8E

Low pH native structure of leucine aminopeptidase from Pseudomonas putida

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-3
Synchrotron site	ESRF
Beamline	ID14-3
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4r
Wavelength(s)	0.9300
Spacegroup name	P 63
Unit cell lengths	116.946, 116.946, 137.899
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	58.000 - 2.750
Rwork	0.212
R-free	0.26700
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	101.535	2.900
High resolution limit [Å]	2.750	2.750
Rmerge	0.353
Number of reflections	27831
<I/σ(I)>	15.4
Completeness [%]	99.5	99.5
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.2	278	11% (w/v) PEG 8000, 0.2 M Na formate, 0.1 M MES-NaOH, pH 5.2, 1 mM NaN3, VAPOR DIFFUSION, HANGING DROP, temperature 278K