3H8A
Crystal structure of E. coli enolase bound to its cognate RNase E recognition domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-07-02 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 103.883, 110.207, 160.267 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.890 - 1.900 |
R-factor | 0.18 |
Rwork | 0.180 |
R-free | 0.22600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1e9i |
RMSD bond length | 0.017 |
RMSD bond angle | 1.570 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.890 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 136948 | |
Completeness [%] | 99.3 | 95.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 291 | 0.1 M HEPES, 1.6 M NH4SO4, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |