3H84
Crystal structure of GET3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2009-03-12 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 219.400, 113.743, 48.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.300 |
| R-factor | 0.22297 |
| Rwork | 0.222 |
| R-free | 0.24700 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.106 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.076 | 0.586 |
| Number of reflections | 54963 | |
| <I/σ(I)> | 32.77 | 2.2 |
| Completeness [%] | 100.0 | 97.3 |
| Redundancy | 6.2 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6 | 298 | 13% PEG 4K, 0.2M Ammonium sulfate, 20% Ethylene Glycol, 0.1% n-octyl-[beta]-D-glucopyranoside, pH 6.0, EVAPORATION, temperature 298K |
