3H7S

Crystal structures of K63-linked di- and tri-ubiquitin reveal a highly extended chain architecture

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-10-11
Detector	ADSC QUANTUM 210
Wavelength(s)	0.980
Spacegroup name	P 43 3 2
Unit cell lengths	105.320, 105.320, 105.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.824 - 2.300
R-factor	0.1986
Rwork	0.196
R-free	0.25320
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.819
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.300
Number of reflections	9366

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH UNDER OIL	5.2	291	0.2 M Zinc acetate, 6% w/v PEG 8000 and 0.1 M Imidazole-Cl pH 6.5, Al's oil, MICROBATCH UNDER OIL, temperature 291K