3H7P
Crystal structure of K63-linked di-ubiquitin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-10-11 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.980 |
Spacegroup name | P 43 3 2 |
Unit cell lengths | 105.530, 105.530, 105.530 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.210 - 1.900 |
R-factor | 0.195 |
Rwork | 0.193 |
R-free | 0.23600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.158 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.900 |
Number of reflections | 16409 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH UNDER OIL | 5.2 | 291 | 0.2 M Cadmium sulfate, 5% w/v PEG 8000 and 0.1 M Imidazole-Cl pH 6.5, Al's oil, MICROBATCH UNDER OIL, temperature 291K |