3H7I
Structure of the metal-free D132N T4 RNase H
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 36.011, 85.858, 57.260 |
Unit cell angles | 90.00, 92.00, 90.00 |
Refinement procedure
Resolution | 35.989 - 1.502 |
R-factor | 0.1809 |
Rwork | 0.180 |
R-free | 0.21300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1TFR native T4 RNase H |
RMSD bond length | 0.012 |
RMSD bond angle | 1.320 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.048 | 0.089 |
Number of reflections | 52759 | |
<I/σ(I)> | 27.9 | 10 |
Redundancy | 5.8 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 200 mM ammonium acetate, 100 mM sodium cacodylate, 12% PEG 8000, 5% 2-methyl-2,4-pentanediol (MPD), pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |