3H6M
Crystal structure of Staphylococcal nuclease variant Delta+PHS V104E at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.026, 60.461, 38.296 |
| Unit cell angles | 90.00, 93.96, 90.00 |
Refinement procedure
| Resolution | 38.205 - 1.700 |
| R-factor | 0.177 |
| Rwork | 0.172 |
| R-free | 0.22200 |
| Starting model (for MR) | 3bdc |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.250 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.5.0063) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.165 | 0.165 |
| Number of reflections | 15538 | |
| <I/σ(I)> | 19.782 | 19.789 |
| Completeness [%] | 99.7 | 99.4 |
| Redundancy | 11.7 | 11.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 30% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
