3H61
Catalytic domain of human Serine/Threonine Phosphatase 5 (PP5c) with two Mn2+ atoms originally soaked with norcantharidin (which is present in the structure in the hydrolyzed form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-01-25 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97550 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 154.499, 41.781, 105.905 |
Unit cell angles | 90.00, 96.96, 90.00 |
Refinement procedure
Resolution | 37.500 - 1.450 |
R-factor | 0.1643 |
Rwork | 0.161 |
R-free | 0.19375 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1s95 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.378 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.4.0067) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.530 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.063 | 0.393 |
Number of reflections | 115060 | |
<I/σ(I)> | 200.1 | 3.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 2.6 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | 10mM Tris-HC, 40% MPD, 20% PEG MME 5000 , pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |