3H56
Met150Leu/Phe312Cys variant of nitrite reductase from Alcaligenes faecalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Collection date | 2004-06-19 |
| Wavelength(s) | 1 |
| Spacegroup name | H 3 |
| Unit cell lengths | 126.724, 126.724, 65.062 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | ? - 1.500 |
| R-factor | 0.17912 |
| Rwork | 0.178 |
| R-free | 0.20437 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.478 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 54872 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.5 | 298 | pH 4.5, VAPOR DIFFUSION, temperature 298K |
