3H51
Crystal structure of Putative calcium/calmodulin dependent protein kinase II association domain (NP_636218.1) from XANTHOMONAS CAMPESTRIS at 1.70 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-02-18 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.91837,0.97901 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 38.832, 80.550, 51.431 |
Unit cell angles | 90.00, 95.59, 90.00 |
Refinement procedure
Resolution | 29.488 - 1.700 |
R-factor | 0.18 |
Rwork | 0.179 |
R-free | 0.21500 |
Structure solution method | MAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.669 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.5) |
Phasing software | SHELX |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.488 | 29.490 | 1.740 |
High resolution limit [Å] | 1.700 | 7.600 | 1.700 |
Rmerge | 0.074 | 0.047 | 0.665 |
Total number of observations | 1157 | 7906 | |
Number of reflections | 33865 | ||
<I/σ(I)> | 6.464 | 13.3 | 1.2 |
Completeness [%] | 97.9 | 94.4 | 97.3 |
Redundancy | 3.2 | 3.1 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 20.0000% PEG-1000, 0.1M TRIS pH 8.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |