3H4Y

Crystal structure of putative chemotaxis protein (YP_009526.1) from DESULFOVIBRIO VULGARIS HILDENBOROUGH at 1.55 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-03-26
Detector	MARMOSAIC 325 mm CCD
Spacegroup name	P 41 21 2
Unit cell lengths	43.820, 43.820, 158.604
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.399 - 1.550
R-factor	0.163
Rwork	0.162
R-free	0.19600
Structure solution method	SAD
RMSD bond length	0.015
RMSD bond angle	1.631
Data reduction software	MOSFLM
Data scaling software	SCALA (3.2.5)
Phasing software	SOLVE
Refinement software	REFMAC (5.5.0053)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.399	29.410	1.590
High resolution limit [Å]	1.550	6.930	1.550
Rmerge	0.113	0.059	0.935
Total number of observations		3992	11765
Number of reflections	23188
<I/σ(I)>	4.79	10	0.8
Completeness [%]	99.1	98.4	97.5
Redundancy	12.3	11.8	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	277	10.0000% MPD, 0.1M Citrate pH 4.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K