3H4S
Structure of the complex of a mitotic kinesin with its calcium binding regulator
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Detector technology | CCD |
Collection date | 2008-04-01 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 118.800, 118.800, 142.100 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.880 - 2.400 |
R-factor | 0.223 |
Rwork | 0.223 |
R-free | 0.22800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1sdm |
RMSD bond length | 0.011 |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.114 | |
Number of reflections | 23623 | |
<I/σ(I)> | 17.9 | 2.8 |
Completeness [%] | 99.6 | 99.4 |
Redundancy | 11 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 30% PEG400, 100 mM TRIS, 200 mM MgCl2, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |