3H4R
Crystal structure of E. coli RecE exonuclease
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-12-09 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97929 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 123.200, 123.200, 67.260 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.430 - 2.800 |
R-factor | 0.29 |
Rwork | 0.290 |
R-free | 0.31300 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.400 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.080 | 0.700 |
Number of reflections | 13172 | |
<I/σ(I)> | 19.6 | 3.3 |
Completeness [%] | 99.4 | 100 |
Redundancy | 23.5 | 23.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 30-42% Glycerol, 100 mM DL-malic acid, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |