3H4R
Crystal structure of E. coli RecE exonuclease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-09 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 123.200, 123.200, 67.260 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.430 - 2.800 |
| R-factor | 0.29 |
| Rwork | 0.290 |
| R-free | 0.31300 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.080 | 0.700 |
| Number of reflections | 13172 | |
| <I/σ(I)> | 19.6 | 3.3 |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 23.5 | 23.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 30-42% Glycerol, 100 mM DL-malic acid, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
