3H4N
PpcD, A cytochrome c7 from Geobacter sulfurreducens
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-08-25 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 1.74041, 1.73818, 1.78832, 1.69077, 1.0332 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 27.400, 55.900, 94.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.040 - 1.350 |
| R-factor | 0.15171 |
| Rwork | 0.149 |
| R-free | 0.17256 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.413 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.390 |
| High resolution limit [Å] | 1.340 | 1.340 |
| Rmerge | 0.047 | 0.147 |
| Number of reflections | 60576 | |
| <I/σ(I)> | 42.1 | 8.4 |
| Completeness [%] | 97.0 | 79 |
| Redundancy | 4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 298 | Crystal obtained from Index screen-95 (30% PEG monomethylether 2000, 0.1 M potassium thiocyanate), pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
