3H4L
Crystal Structure of N terminal domain of a DNA repair protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-06-10 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.592, 103.288, 117.167 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.870 - 2.500 |
R-factor | 0.235 |
Rwork | 0.235 |
R-free | 0.28600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hs7 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MLPHARE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 30119 | |
<I/σ(I)> | 17 | 2.2 |
Completeness [%] | 97.7 | 99.4 |
Redundancy | 3.4 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Batch | 8.5 | 277 | .1M Tris, 10% PEG 4K, 0.2M sodium acetate, pH 8.5, Batch, temperature 277K |