3H4H
Met94Thr/Phe312Cys variant of nitrite reductase from Alcaligenes faecalis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Collection date | 2004-06-19 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.301, 102.077, 146.336 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.600 |
R-factor | 0.18281 |
Rwork | 0.181 |
R-free | 0.22594 |
Structure solution method | Refinement of wild-type structure |
RMSD bond length | 0.012 |
RMSD bond angle | 1.386 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 84.520 |
High resolution limit [Å] | 1.600 |
Number of reflections | 103196 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |